Nitric oxide radical scavenging activity of
Estimation of total phenolic content by spectrophotometer By Folin—Denis method The method is based on the oxidation of molecule containing —OH groups. Plant material Leaves of T.
Nitric oxide assay principle
However, it is unknown whether oxidative stress is the cause or the consequence of disease. The Total Phenolic Content was expressed as mg tannic acid equivalents per g dry weight of sample. Table 1. Antioxidants are also widely used as ingredients in dietary supplements in the hope of maintaining health and preventing diseases such as cancer and coronary heart disease. Results The yield of the ethanolic leaf extract of N. In sub-Saharan Africa, the plant is used in the management of a variety of ailments for example, the bark is chewed and swallowed for stomach pains and diarrhoea as well as toothache [ 5 ]. Newbouldia laevis is commonly known as African border tree. Oxidation is a chemical reaction that transfers electrons from a substance to an oxidizing agent. The study therefore, evaluated the principal metabolites present in the ethanolic leaf extract of the plant as well as the antioxidant potential and free radical scavenging activity of the leaf extract. The plant material was authenticated at the Department of Botany, Govt.
Method: The therapeutic effects of tannins and flavonoids can be largely attributed to their antioxidant properties. This research work was undertaken to investigate the in vitro antioxidant activity of aqueous and ethanolic extracts of the leaves.
Superoxide radical scavenging activity of plant extracts
Keywords: Ferric reducing antioxidant power, 1, 1-diphenylpicrylhydrazyl-radical scavenging assay, Tephrosia purpurea Linn. This study was undertaken using the spectrophotometric method. This drug is also not official in Ayurvedic Pharmacopoeia. In conclusion, T. However, it is unknown whether oxidative stress is the cause or the consequence of disease. Phytochemical analysis Preliminary phytochemical screening of N. Arts and Science College, Modasa, Gujarat.
The leaf parts were chopped and dried at room temperature for 10 days and used as raw material. The preconditioned set method can be optimized quantitatively by evaluating the presence of any constituent like tannins, flavonoids or any marker, by suitable validation methods.
Ascorbic acid dpph radical scavenging activity
Method: The therapeutic effects of tannins and flavonoids can be largely attributed to their antioxidant properties. Results Chromatographic and spectrophotometric methods were used in the study and modified where necessary in the study. Result: The total phenolic content of aqueous and ethanolic extracts showed the content values of 9. This article has been cited by other articles in PMC. To each flask, 0. Preparation of raw material Preparation of crude aqueous extract Accurately g of powder was weighed accurately and taken in a stainless steel vessel and mixed with ml of distilled water. Received Sep 4; Accepted Feb Department of Pharmacognosy and Phytochemistry, Shri B. Evaluation of bioactive metabolic constituents gave high levels of ascorbic acid
Leguminosae leaves possess the antioxidant substance which may be responsible for the treatment of jaundice and other oxidative stress-related diseases. The yield of the ethanolic leaf extract of N.
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